Detection of GAD65 Autoreactive T-Cells by HLA Class I Tetramers in Type 1 Diabetic Patients
Figure 2
Density plots of cytometric analysis of PBMC of one representative T1D patient at day 6 of culture. (a), (b), (c) Cells stimulated with IL-2; (d), (e), (f) cells stimulated with the GAD65 peptide. (a), (d) Density Plots of morphological parameters (FSC-H; SSC-H) of cells treated with IL-2 and GAD65, respectively. In gate R1 cells were selected by morphological parameters as living cells. In the subsequent panels ((b), (c), (e), (f)) only this region was considered. ((b), (e)) Density Plots of CD3 APC and CD8 FITC cells, treated with IL-2 and GAD65 peptide, respectively. Positive CD3/CD8 cells were selected in R2 gate. In (c) and (f) panel only R2 gate was considered and the percentage in the upper right quadrant represented CD3+/CD8+/GAD65 reactive T lymphocytes.
