Methodology Report
Detection of GAD65 Autoreactive T-Cells by HLA Class I Tetramers in Type 1 Diabetic Patients
Table 1
Sex, age, islet-related autoantibody (IA-2A, IAA and GADA) profile, and associated disorders in 9 newly diagnosed T1D patients at disease onset. The diagnosis of T1D was done according to the WHO and the American Diabetes association (ADA) criteria. NT: not tested; pos: positive; neg: negative.
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Assay performed at San Raffaele Hospital, Milan, Italy. GADA and IA-2A measurements were performed by radiobinding assay with in vitro translated 35S-methionine-labeled GAD65 or IA-2 [31, 32]. IAA were measured using a competitive protein A/G insulin radiobinding assay [33]. |