Figure 6: Validation of changes in TLR-4 (73 KD) and NF-B p65 (80 kDa) protein expression by western blotting. Proteins extracted were prepared from arteries () of mice subjected to CMS or normal conditions for 0, 4, and 12 weeks, respectively. Each lane shows representative western blots using anti-TLR4 or NF-B p65 and anti-GAPDH bodies in (a1) and (a2), respectively. Each panel summarizes densitometric readings of band intensities normalized to GAPDH, which was measured by densitometry with Image J image analysis software. (b1), (b2) densitometric measurements TLR4 and NF-B p65 from Western blots, respectively. Data are mean ± SEM. C: control group; S: chronic mild stress group ( per group). *P , **P compared with the control. The data are representative of three experiments.