Nonviral Production of Human Interleukin-7 in Spodoptera Frugiperda Insect Cells as a Soluble Recombinant Protein
Figure 2
Screening of puromycin resistant clones in Sf9 cells co-transfected with pIE1/153A (V4)
expression plasmid carrying hIL-7 gene and pBmA-pac carrying puromycin resistance gene. Cells
were placed in medium containing puromycin at a final concentration of 5 gmL−1 for 3 weeks.
Lane 1: protein marker. Lanes 2–11: polyclones Sf9hIL-7G, Sf9hIL-7E, Sf9hIL-7F, Sf9hIL-7A1,
Sf9hIL-7B4, Sf9hIL-7D5, Sf9hIL-7H, Sf9hIL-7C1-2, Sf9hIL-7C1-3, and Sf9hIL-B4-1, respectively.