Nonviral Production of Human Interleukin-7 in Spodoptera Frugiperda Insect Cells as a Soluble Recombinant Protein

Figure 8

Western blot analysis of the intra- and extracellular of rhIL-7 produced in Sf9 insect cells. (a) Intracellular protein in Sf9: (lanes 1–3: 24 hpi, using MOI 5, 1 and 0.5 of the virus stock, respectively, lanes 4–6 48 hpi. Lanes 7–9: 72 hpi and lanes 10–12 96 hpi). (b) Extracellular protein in Sf9: (lanes: 2–4 24 hpi using MOI 5, 1 and 0.5 virus stock, respectively, lanes: 5–7 48 hpi, lanes: 8–10 72 hpi, lanes: 11–13 96 hpi).