An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum

Figure 5

PCR identification of the  SP  Δ𝑎𝑠𝑑   (Cm)   mutants.

(a) Overall of PCR identification of the  SP  Δ𝑎𝑠𝑑   (Cm)   mutant Lanes 1, 2, 5, 6, 7, 8, 10, 11: the  SP  Δ𝑎𝑠𝑑   (Cm) mutant; Lanes 3, 4, 9, 12:wt, Lanes 1, 2,3: 𝐶𝑚^F/𝐶𝑚^R; 4, 5, 6: asdP5/asdP; 6, 7, 8, 9: asdP1/asdP2; 10, 11, 12: asdP3/asdP4

(b) PCR with primers asdP5/asdP6 identified Wt,   SP  Δ𝑎𝑠𝑑   (Cm)   and  SP  Δ𝑎𝑠𝑑 mutant, Lane 1: wt; Lanes 2, 3: First crossover of conjugants; Lanes 4, 5: Second crossover of conjugants (the  SP  Δ𝑎𝑠𝑑   (Cm) mutant); Lanes 6, 7: the  SP  Δ𝑎𝑠𝑑 mutant.