An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum

Table 2

The primer sequences used for PCR amplification.

Gene amplified	Primers	Primer sequences (5^′-3^′)	Amplicon size (bp)	Note
Upstream of asd	asdp1	ttggatccccgttgaatgatgatgaccg	1959	BamH I
	asdp2	ttctcgagtgcgttaggaagggaatc		Xho I
Downstream of asd	asdp3	ttctcgaggtagcttaatcccgcgggta	2079	Xho I
	asdp4	ttggatccgagcgttcattgtcatcgac		BamH I
asd	asdp5	ttgcttccaactgctgagc	1796(wt)
	asdp6	tcctatctgcgtcgtcctac	1360(Δ𝑎𝑠𝑑 + Cm)
𝐶𝑚^R	𝐶𝑚^F	actcgaggtgtaggctggagctgcttc	1032	Xho I
	𝐶𝑚^R	actcgagatgggaattagccatggtcc		Xho I
rfbS	rfbSF	tgcctatcagagtattagtgt	400	S.Pullorum
	rfbSR	tattcacgaattgatatatcc
hto	htoF	actggcgttatccctttctctgctg	495	genus Salmonella
	htoR	atgttgtcctgcccctggtaagaga