An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum

Table 3

Antibiotics resistance of bacteria during the selection of Δ𝐚𝐬𝐝 mutant.

Bacteria	Antibiotics
	Nal	Km	Cm	Sm	Amp
Salmonella Pullorum (S06004)	+	−	−	−	−
𝜒7213(pGMB151-Δ𝑎𝑠𝑑/Cm)	−	+	+	+	+
First crossover (S06004::pGMB151-Δ𝑎𝑠𝑑/Cm )	+	−	+	+	+
Second crossover (Δ𝑎𝑠𝑑 (Cm) mutant)	+	−	+	−	−
SP  Δasd  mutant	+	−	−	−	−