Virus-Specific Read-Through Codon Preference Affects Infectivity of Chimeric Cucumber Green Mottle Mosaic Viruses Displaying a Dengue Virus Epitope
Figure 1
Position of primers in
constructed plasmid clones. Primers 4–8 were used to amplify the EB4 gene from
pCANTAB 5E. Plasmid pCGT7X and amplified EB4 fragments were digested with HindIII and ligated together to obtain
the respective plasmid clones as shown. HindIII
is the insertion site of EB4 at the end of CGMMV CP. PCR amplification using
primer pairs 1–9, 3–9, and 2–7 will yield amplified
products of approximately 6.5 kb, 0.85 kb, and 2.2 kb, respectively.