Research Article

Virus-Specific Read-Through Codon Preference Affects Infectivity of Chimeric Cucumber Green Mottle Mosaic Viruses Displaying a Dengue Virus Epitope

Figure 1

Position of primers in constructed plasmid clones. Primers 4–8 were used to amplify the EB4 gene from pCANTAB 5E. Plasmid pCGT7X and amplified EB4 fragments were digested with HindIII and ligated together to obtain the respective plasmid clones as shown. HindIII is the insertion site of EB4 at the end of CGMMV CP. PCR amplification using primer pairs 1–9, 3–9, and 2–7 will yield amplified products of approximately 6.5 kb, 0.85 kb, and 2.2 kb, respectively.
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