Research Article

Virus-Specific Read-Through Codon Preference Affects Infectivity of Chimeric Cucumber Green Mottle Mosaic Viruses Displaying a Dengue Virus Epitope

Figure 7

Sequence alignments of cloned RT-PCR-amplified products from putative chimeric CGMMV RNA at 30 dpi. Plasmid clones sent for sequence analysis were pR_P3U1, pR_P3U3, pR_P3U4, and pR_P3U11. The sequence alignment shows that EB4 sequence was not within the insert of plasmids, except for a partial sequence for pR_P3U4 clone. Only part of the downstream sequence of EB4 was detected together with untranslated region of CGMMV for pR_P3U4 clone, the rest were without EB4 sequence. Introduced read-through sequences and extra codons are underlined. Complete EB4 sequence is aligned accordingly with the other sequences.
781712.fig.007