Research Article
Virus-Specific Read-Through Codon Preference Affects Infectivity of Chimeric Cucumber Green Mottle Mosaic Viruses Displaying a Dengue Virus Epitope
Table 2
Amino acid sequence, isoelectric
point (pI) and charge of wild type, constructed recombinant CGMMV coat protein
(CP), and the EB4 insert.
| Clones | Amino acid sequence | MW | pI of CP | Charge | (kDa) | of CP |
| EB4 | IIGVEPGQLKLNWFKKGSSIGQMIETTMRGAKRMAILGDT
AWDFG | 5.0 | 9.53 | +1.9 |
| | MAYNPITPSKLIAFSASYVPVRTLLNFLVASQGTAFQTQAGRD
| 17.3 | 5.08 | −3.1 | Wild-type | SFRESLSALPSSVVDINSRFPDAGFYAFLNGPVLRPIFVSLLSST
| CGMMV | DTRNRVIEVVDPSNPTTAESLNAVKRTDDASTAARAEIDNLIE
| | SISKGFDVYDRASFEAAFSVVWSEATTSKA |
| pRT* | MAYNPITPSKLIAFSASYVPVRTLLNFLVASQGTAFQTQAGRD
| 23.0 | 5.41 | −2.1 | SFRESLSALPSSVVDINSRFPDAGFYAFLNGPVLRPIFVSLLSST
| DTRNRVIEVVDPSNPTTAESLNAVKRTDDASTAARAEIDNLIE
| SISKGFDVYDRASFEAAFSVVWSEATTSKACQQLIIGVEPGQ | LKLNWFKKGSSIGQMIETTMRGAKRMAILGDTAWDFGEA |
| pRG* | MAYNPITPSKLIAFSASYVPVRTLLNFLVASQGTAFQTQAGRD
| 23.1 | 5.69 | −1.1 | SFRESLSALPSSVVDINSRFPDAGFYAFLNGPVLRPIFVSLLSST
| DTRNRVIEVVDPSNPTTAESLNAVKRTDDASTAARAEIDNLIE
| SISKGFDVYDRASFEAAFSVVWSEATTSKAWQQLIIGVEPGQ | LKLNWFKKGSSIGQMIETTMRGAKRMAILGDTAWDFGQA |
| pCG+FSRTRE* | MAYNPITPSKLIAFSASYVPVRTLLNFLVASQGTAFQTQAGRD
| 22.9 | 5.41 | −2.0 | SFRESLSALPSSVVDINSRFPDAGFYAFLNGPVLRPIFVSLLSST
| DTRNRVIEVVDPSNPTTAESLNAVKRTDDASTAARAEIDNLIE
| SISKGFDVYDRASFEAAFSVVWSEATTSKASGQLIIGVEPGQ | LKLNWFKKGSSIGQMIETTMRGAKRMAILGDTAWDFGEA |
|
|
ith
read-through leaky UAG amber stop codon.
|