Research Article

Virus-Specific Read-Through Codon Preference Affects Infectivity of Chimeric Cucumber Green Mottle Mosaic Viruses Displaying a Dengue Virus Epitope

Table 2

Amino acid sequence, isoelectric point (pI) and charge of wild type, constructed recombinant CGMMV coat protein (CP), and the EB4 insert.

ClonesAmino acid sequenceMW pI of CPCharge
(kDa)of CP

EB4IIGVEPGQLKLNWFKKGSSIGQMIETTMRGAKRMAILGDT AWDFG 5.09.53+1.9

MAYNPITPSKLIAFSASYVPVRTLLNFLVASQGTAFQTQAGRD 17.35.08−3.1
Wild-type SFRESLSALPSSVVDINSRFPDAGFYAFLNGPVLRPIFVSLLSST
CGMMVDTRNRVIEVVDPSNPTTAESLNAVKRTDDASTAARAEIDNLIE
SISKGFDVYDRASFEAAFSVVWSEATTSKA

pRT*MAYNPITPSKLIAFSASYVPVRTLLNFLVASQGTAFQTQAGRD 23.05.41−2.1
SFRESLSALPSSVVDINSRFPDAGFYAFLNGPVLRPIFVSLLSST
DTRNRVIEVVDPSNPTTAESLNAVKRTDDASTAARAEIDNLIE
SISKGFDVYDRASFEAAFSVVWSEATTSKACQQLIIGVEPGQ
LKLNWFKKGSSIGQMIETTMRGAKRMAILGDTAWDFGEA

pRG*MAYNPITPSKLIAFSASYVPVRTLLNFLVASQGTAFQTQAGRD 23.15.69−1.1
SFRESLSALPSSVVDINSRFPDAGFYAFLNGPVLRPIFVSLLSST
DTRNRVIEVVDPSNPTTAESLNAVKRTDDASTAARAEIDNLIE
SISKGFDVYDRASFEAAFSVVWSEATTSKAWQQLIIGVEPGQ
LKLNWFKKGSSIGQMIETTMRGAKRMAILGDTAWDFGQA

pCG+FSRTRE*MAYNPITPSKLIAFSASYVPVRTLLNFLVASQGTAFQTQAGRD 22.95.41−2.0
SFRESLSALPSSVVDINSRFPDAGFYAFLNGPVLRPIFVSLLSST
DTRNRVIEVVDPSNPTTAESLNAVKRTDDASTAARAEIDNLIE
SISKGFDVYDRASFEAAFSVVWSEATTSKASGQLIIGVEPGQ
LKLNWFKKGSSIGQMIETTMRGAKRMAILGDTAWDFGEA

ith read-through leaky UAG amber stop codon.