High Harvest Yield, High Expansion, and Phenotype Stability of CD146 Mesenchymal Stromal Cells from Whole Primitive Human Umbilical Cord Tissue

Table 1

Physical properties and cell distributions are used to calculate the theoretical cell numbers in each location. Measurements (mean ± st. dev.) are based on fixed, frozen sections and as shown in Figure 1 and Supplemental Table 1.

Parameter		Measurement
(1) Mean length of human umbilical cord (cm)		30–60
(2) Mean diameter of human umbilical cord (cm)		1.3 ±0.25
(3) Arterial diameter (𝜇m)		2040 ±66
(4) Vein diameter (𝜇m)		3550 ±190
(5) Perivascular region, radial distance (thickness, 𝜇m)		4230 ±120
Potential cell harvest yields
Vessels,	Theoretical Total Number of Cells per gram	8.1–8.9 × 10^6
Perivascular regions,	Theoretical Total Number of Cells per gram	~1 × 10^6
Wharton’s jelly,	Theoretical Total Number of Cells per gram	1.3–1.4 × 10^6