DNA Damage/Repair and Polymorphism of the hOGG1 Gene in Lymphocytes of AMD Patients

Table 1

Repair of hydrogen peroxide-induced DNA damage in 30 AMD patients and 30 healthy controls. DNA damage for each treatment was analyzed as the percentage of DNA in the tail of 100 comets; mean ± S.E.M. The number of cells scored in each treatment (patient) was 100. The efficacy of DNA repair was calculated by comparing the extent of DNA damage immediately and 120 minutes after hydrogen peroxide treatment. The subjects with no significant repair are presented bold.

No	Age (yrs)/Sex	Control DNA damage ± S.EM.		Efficacy of DNA repair (%)	P	No	Age (yrs)/Sex	AMD DNA damage ± S.EM.		Efficacy of DNA repair (%)	𝑃
		0^′	120^′					0^′	120^′
1	72/W	12.19 ± 4.13	5.62 ± 2.34	53.90	> .05	1	72/M	7.30 ± 0.88	1.29 ± 0.27	82.33	< .001
2	60/M	15.73 ± 2.12	5.84 ± 1.23	62.87	< .001	2	60/M	6.43 ± 0.62	1.44 ± 0.19	77.61	< .001
3	81/W	15.89 ± 2.09	9.02 ± 1.42	43.24	< .01	3	61/M	4.38 ± 0.50	6.66 ± 0.84	−𝟓𝟐.𝟎𝟓	< .05
4	77/W	15.99 ± 2.87	8.95 ± 2.15	44.03	> .05	4	64/W	4.85 ± 0.50	2.00 ± 0.35	58.76	< .001
5	59/W	12.49 ± 1.90	5.60 ± 2.99	55.17	> .05	5	65/M	3.73 ± 0.41	6.16 ± 1.07	−𝟔𝟓.𝟏𝟓	< .05
6	64/W	9.78 ± 1.24	4.48 ± 2.12	54.19	< .05	6	82/W	3.66 ± 0.38	0.83 ± 0.17	77.32	< .001
7	80/W	20.01 ± 2.30	9.34 ± 1.57	53.32	< .001	7	59/M	7.59 ± 1.37	6.67 ± 1.37	12.13	> .05
8	60/M	12.51 ± 1.38	4.17 ± 0.49	66.67	< .001	8	70/M	17.57 ± 1.41	6.66 ± 2.20	62.10	< .001
9	73/M	6.89 ± 1.73	3.26 ± 0.74	52.69	> .05	9	78/W	12.37 ± 1.92	10.48 ± 1.97	15.28	> .05
10	72/W	6.73 ± 0.88	4.26 ± 0.98	36.70	> .05	10	79/W	2.51 ± 0.40	4.04 ± 0.38	−𝟔𝟎.𝟗𝟔	< .05
11	66/M	11.18 ± 2.17	6.21 ± 1.53	44.46	> .05	11	73/W	6.26 ± 0.99	8.33 ± 1.54	−𝟑𝟑.𝟎𝟕	> .05
12	80/W	13.92 ± 4.11	6.47 ± 2.21	53.52	> .05	12	60/W	3.19 ± 0.45	4.46 ± 0.87	−𝟑𝟗.𝟖𝟏	> .05
13	60/M	8.95 ± 1.46	2.04 ± 0.40	77.21	< .001	13	63/W	6.81 ± 1.14	3.53 ± 1.93	48.17	> .05
14	79/W	10.11 ± 1.80	8.76 ± 2.04	13.35	> .05	14	73/W	3.81 ± 0.61	3.24 ± 0.39	14.96	> .05
15	64/W	14.52 ± 1.36	6.13 ± 0.92	57.78	<.001	15	70/M	7.41 ± 0.64	5.14 ± 0.56	30.64	< .05
16	75/M	4.12 ± 0.65	3.63 ± 0.58	11.89	> .05	16	74/W	9.40 ± 1.56	3.15 ± 0.49	66.49	< .001
17	73/W	14.63 ± 1.38	5.19 ± 0.67	64.53	< .001	17	75/W	9.59 ± 1.58	12.53 ± 1.81	−𝟑𝟎.𝟔𝟔	> .05
18	69/M	18.29 ± 1.28	6.60 ± 1.12	63.92	> .05	18	64/M	9.35 ± 1.36	7.56 ± 0.97	19.15	> .05
19	66/M	17.46 ± 4.25	8.80 ± 1.58	49.60	> .05	19	77/W	2.14 ± 0.23	2.48 ± 0.48	−𝟏𝟓.𝟖𝟗	> .05
20	77/W	18.19 ± 4.16	8.63 ± 3.20	52.56	> .05	20	62/W	12.00 ± 1.32	14.58 ± 0.85	−𝟐𝟏.𝟓𝟎	> .05
21	75/M	9.55 ± 1.07	7.05 ± 2.58	26.18	> .05	21	69/M	10.11 ± 0.80	17.34 ± 0.89	−𝟕𝟏.𝟓𝟏	< .001
22	64/M	11.31 ± 2.09	9.35 ± 2.50	17.33	> .05	22	74/M	34.88 ± 2.65	26.54 ± 1.43	23.91	< .01
23	70/W	13.53 ± 2.18	7.91 ± 1.52	41.54	< .05	23	72/M	8.63 ± 0.89	18.09 ± 1.23	−𝟏𝟎𝟗.𝟔𝟐	< .001
24	63/W	14.39 ± 3.60	7.41 ± 1.18	48.51	> .05	24	80/W	34.05 ± 4.39	25.75 ± 2.97	24.38	> .05
25	71/M	12.32 ± 2.12	5.31 ± 1.72	56.90	< .05	25	80/W	52.14 ± 3.41	19.01 ± 1.71	63.54	< .001
26	72/W	8.37 ± 0.87	3.67 ± 0.33	56.16	< .001	26	69/W	12.71 ± 1.20	21.93 ± 2.30	−𝟕𝟐.𝟓𝟒	< .001
27	70/M	12.32 ± 2.12	5.31 ± 1.72	56.90	< .05	27	72/M	5.14 ± 0.46	8.13 ± 0.77	−𝟓𝟖.𝟏𝟕	< .01
28	71/M	14.39 ± 2.60	7.41 ± 1.28	48.51	> .05	28	69/M	17.02 ± 1.59	23.14 ± 2.03	−𝟑𝟓.𝟗𝟔	< .05
29	73/M	12.53 ± 2.17	7.91 ± 1.22	36.87	< .05	29	73/W	18.22 ± 2.75	15.72 ± 1.13	13.72	> .05
30	70/W	11.31 ± 2.19	9.35 ± 2.25	17.33	> .05	30	66/M	13.48 ± 1.59	12.16 ± 1.82	9.79	>.05
Mean	70 ± 6	12.65 ± 0.66	6.46 ± 0.37	47.26 ± 2.92		Mean	70 ± 7	11.56 ± 1.98	9.97 ± 1.39	1.11 ± 9. 56