Functional Expression of a DNA-Topoisomerase IB from Cryptosporidium parvum
Figure 4
Expression of CpTopIB gene induces sensitivity to CPT-resistant yeast strain MBY3 (rad52 ). Exponentially growing cells in dextrose, transformed with vectors carrying the cryptosporidial wild type and point mutations driven by GAL1 promoter, were serially 10-fold diluted starting from OD595 = 0.5. Five microliters were spotted on selective media under induced (2% galactose) conditions in the presence or absence of 30 M CPT. All plates contained 0.375% DMSO. Empty assay was performed under similar assay conditions but expression the YCpGAL(leu-) vector without any insert.
