Research Article
Functional Expression of a DNA-Topoisomerase IB from Cryptosporidium parvum
Table 1
Sequences of the primers used in this study to clone and to mutate CpTopIB.
| Specimen | | Primers (-) |
| Wild type | FW | ATA AGA ATG CGG CCG CAT GGC ATC AGC TAC GGA TTC | RV | GGA CTA GTT TAA AAT GAA AAA TCT GAT CTC G | R318A | FW | GAT TTT TTA GCT TTG GCG GTC GGA GGA GAA AAA G | RV | C TTT TTC TCC TCC GAC CGC CAA AGC TAA AAA ATC | K358A | FW | CTT TAG ATT TTT TGG GCG CGG ATT CAA TTC GAT AC | RV | GT ATC GAA TTG AAT CCG CGC CCA AAA AAT CTA AAG | R416A | FW | CT GCC AAG G TA TTT GCG ACA TTC AAT GCA TC | RV | GA TGC ATT GAA TGT CGC AAA TAC CTT GGC AG | H489A | FW | GCG ATA TTA TGT AAC GCG CAG AGA TCA GTT CC | RV | GG AAC TGA TCT CTG CGC GTT ACA TAA TAT CGC | Y600F | FW | CA TCG AAG ATT AAC TTT ATG GAT CCC AGG ATT TC | RV | GA AAT CCT GGG ATC CAT AAA GTT AAT CTT CGA TG |
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