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Journal of Biomedicine and Biotechnology
Volume 2009 (2009), Article ID 875629, 8 pages
http://dx.doi.org/10.1155/2009/875629
Research Article

Separation and Purification of Two Flavone Glucuronides from Erigeron multiradiatus (Lindl.) Benth with Macroporous Resins

1Ethnic Pharmaceutical Institute, Southwest University for Nationalities, Chengdu 610041, China
2West China School of Pharmacy, Sichuan University, Chengdu 610041, China

Received 21 March 2009; Revised 30 June 2009; Accepted 19 August 2009

Academic Editor: H. M. Häggman

Copyright © 2009 Zhi-feng Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Scutellarein-7---D-glucuronide (SG) and apigenin-7---D-glucuronide (AG) are two major bioactive constituents in Erigeron multiradiatus. In this study, a simple method for preparative separation of the two flavone glucuronides was established with macroporous resins. The performance and adsorption characteristics of eight macroporous resins including AB-8, HPD100, HPD450, HPD600, D100, D101, D141 and D160 had been evaluated. The results confirmed that D141 resin was preferred choice, which offered the best adsorption and desorption capacities for the two glucuronides among the tested resins. Sorption isotherms were constructed for D141 resin and fitted well to the Freundlich and Langmuir models (). After one run treatment with D141 resin, the two constituents' content was increased from 2.14% and 1.34% in the crude extract of E. multiradiatus to 24.63% and 18.42% in the final products with the recoveries of 82.5% and 85.4%, respectively. The enrichment of SG and AG can be easily and effectively achieved via adsorption and desorption on D141 resin, and the method can be referenced for large-scale separation and purification of flavone glucuronides from herbal raw materials.