Interfacing Sca- Mesenchymal Stem Cells with Biocompatible Scaffolds with Different Chemical Composition and Geometry
Figure 2
mTERT-MSC line retains morphology, phenotype, and multilineage capacity of Sca- MSC. (a) Freshly extracted (p1) Sca- MSC show typical spindle-shaped morphology (see inset), while (b) after 10 passages in culture they proceed to senescence and fail to grow as monolayer; (c) mTERT-MSC preserve the ability to grow in a monolayer. Bar; 20 m; (d, e) semiquantitative RT-PCR and FACS analyses showing that mTERT ectopic expression preserves typical Sca- MSC stem cell phenotype; (f) thorough characterization of mTERT-MSC phenotype; (g) comparison between mTERT-MSC and MSC multilineage potential in culture (Bars: 100 m).