Gene Expression Analysis of an <i>EGFR</i> Indirectly Related Pathway Identified <i>PTEN</i> and <i>MMP9</i> as Reliable Diagnostic Markers for Human Glial Tumor Specimens

<table class="table-group" id="tab5"><tr><td><table class="table"><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr class="thead"><td align="left">Gene</td><td align="center">Accession number</td><td align="center">PCR primer sequences (<svg height="17.9125" id="M37" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 12.4375 17.9125" width="12.4375" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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l-26 -65h-233z" id="x35"></path></g>
<g transform="matrix(.012,-0,0,-.012,8.225,9.488)"><path d="M227 744l-123 -338l-31 15l73 368q12 3 41.5 -8t36.5 -20z" id="x2032"></path></g>
</svg>-<svg height="17.9125" id="M38" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 12.4375 17.9125" width="12.4375" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,17.65)"><path d="M285 378v-2q65 -13 102 -54.5t37 -97.5q0 -57 -30.5 -104.5t-74 -75t-85.5 -42t-72 -14.5q-31 0 -59.5 11t-40.5 23q-19 18 -16 36q1 16 23 33q13 10 24 0q58 -51 124 -51q55 0 88 40t33 112q0 64 -39 96.5t-88 32.5q-29 0 -64 -11l-6 29q77 25 118 57.5t41 84.5
q0 45 -26.5 69.5t-68.5 24.5q-67 0 -120 -79l-20 20l43 63q51 56 127 56h1q66 0 107 -37t41 -95q0 -42 -31 -71q-22 -23 -68 -54z" id="x33"></path></g>
<g transform="matrix(.012,-0,0,-.012,8.225,9.488)"><path d="M227 744l-123 -338l-31 15l73 368q12 3 41.5 -8t36.5 -20z" id="x2032"></path></g>
</svg>)</td><td align="center">PCR product (bp)</td></tr><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr><td align="left" rowspan="2"><i>EGFR</i></td><td align="center" rowspan="2">NM_005228</td><td align="center">AGGAAGAAGCTTGCTGGTAGC</td><td align="center" rowspan="2">88 </td></tr><tr><td align="center">CTCTGGAAGACTTGTGGCTTG</td></tr><tr><td align="left" rowspan="2"><i>ADAM17</i></td><td align="center" rowspan="2">NM_003183</td><td align="center">CAAGTCATTTGAGGATCTCACG</td><td align="center" rowspan="2">96 </td></tr><tr><td align="center">TCTTTGCTGTCAACACGATTCT</td></tr><tr><td align="left" rowspan="2"><i>HB-EGF</i></td><td align="center" rowspan="2">NM_001945</td><td align="center">GCCTAGGCGATTTTGTCTACC</td><td align="center" rowspan="2">119 </td></tr><tr><td align="center">GCCCAACCTCTTCTGAGACTT</td></tr><tr><td align="left" rowspan="2"><i>PTEN</i></td><td align="center" rowspan="2">NM_000314</td><td align="center">CAGCAGTGGCTCTGTGTGTAA</td><td align="center" rowspan="2">98 </td></tr><tr><td align="center">ATGGACATCTGATTGGGATGA</td></tr><tr><td align="left" rowspan="2"><i>MMP9</i></td><td align="center" rowspan="2">NM_004994</td><td align="center">AAAGCCTATTTCTGCCAGGAC</td><td align="center" rowspan="2">105 </td></tr><tr><td align="center">GCACTGCAGGATGTCATAGGT</td></tr><tr class="table-tr"><td colspan="4"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

GeneBank accession numbers, PCR primer sequences, and products.

BioMed Research International

tab5

Table 5

Table 5: Gene Expression Analysis of an <i>EGFR</i> Indirectly Related Pathway Identified <i>PTEN</i> and <i>MMP9</i> as Reliable Diagnostic Markers for Human Glial Tumor Specimens 

Table 5 | Gene Expression Analysis of an EGFR Indirectly Related Pathway Identified PTEN and MMP9 as Reliable Diagnostic Markers for Human Glial Tumor Specimens 