Quantification of Spatial Parameters in 3D Cellular Constructs Using Graph Theory
Figure 3
Changes in cell density alter compaction and nuclear organization. hMSC at a cell density of 1 106 cells/mL embedded in 2 mg/mL collagen I gel. Collagen I gels were fixed and then stained for nuclei with SYTOX Green. (a) Macroscale images of hydrogel compaction (Scale bar 1 cm). (b) Fluorescent image cross section of hMSC nuclei (Scale bar 100 m).
