About this Journal Submit a Manuscript Table of Contents
Journal of Biomedicine and Biotechnology
Volume 2009 (2009), Article ID 932961, 7 pages
Research Article

Immunohistochemical Localisation of PDE5 in Rat Lung during Pre- and Postnatal Development

1Department of Cellular and Developmental Biology, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy
2Department of Basic and Applied Biology, University of L'Aquila, Via Vetoio 10, 67010 L'Aquila, Italy

Received 31 March 2009; Accepted 18 June 2009

Academic Editor: Richard Tucker

Copyright © 2009 Angela Scipioni et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In mammalian lung, at the transition to extrauterine life, NO/cGMP signal transduction system is known to play crucial roles in the regulation of vascular resistance and is supposed to act in angiogenesis. PDE5, which is the most abundant cGMP metabolizing enzyme within the lung, is highly expressed in the perinatal period, but its localisation in the different pulmonary cells is still poorly known. In our research, PDE5 immunohistochemical distribution was investigated in foetal and neonatal rat lung. The highest expression of PDE5 was found in cells randomly located in the stroma; in newborns, in particular, many cells in the intersaccular walls were heavily labelled, while much lower staining levels were shown by smooth myocytes belonging to vessels and airways. On the basis of their immunoreactivity for š¯›¼ -SM actin and/or desmin, most of the heavily PDE5-positive cells were identified as interstitial myofibroblasts and transitional pericytes, while only a few were interpreted as interstitial lipofibroblasts.