Novel miR390-Dependent Transacting siRNA Precursors in Plants Revealed by a PCR-Based Experimental Approach and Database Analysis
Figure 1
Agarose gel analysis of PCR products. (a) Amplification of genomic DNA sequences flanked by miR390 sites. PCR products were obtained on Nicotiana tabacum genomic DNA with primers P-Tas3 and M-Tas3/caa (Lane 1), on N. tabacum genomic DNA with primers P-Tas3 and M-Tas3/aca (Lane 2), on Arabidopsis thaliana genomic DNA with primers P-Tas3 and M-Tas3/caa (Lane 3), and on A. thaliana genomic DNA with primers P-Tas3 and M-Tas3/aca (Lane 4). Arrow points to the 170-nt PCR product. M is DNA size markers. Marker fragment sizes in base pairs are indicated on the left. (b) Amplification of N. tabacum genomic DNA and cDNA preparation. PCR products were obtained on N. tabacum genomic DNA with primers P-Tas3 and M-Tas3/caa (Lane 1), on N. tabacum cDNA with primers P-Tas3 and M-Tas3/caa (Lane 2), and on N. tabacum cDNA with primers P-Tas3 and M-Tas3/aca (Lane 3). M is DNA size markers. Marker fragment sizes in base pairs are indicated on the left.
