Novel miR390-Dependent Transacting siRNA Precursors in Plants Revealed by a PCR-Based Experimental Approach and Database Analysis
Figure 2
Nucleotide sequence alignment of “280-nt-long” ta-siARF loci of dicot plants. Sequences of Nicotiana tabacum and Nicotiana benthamiana (undelined) were determined in this study, the others were from GeneBank. Boxes indicate miR390 complementary sites and ta-siARF-encoding sequence regions. The length of nonconserved sequence between the -miR390 complementary site and ta-siARF sequence not included in the alignment is indicated. Shading shows residues identical for the given position in most sequences. Sequences of N. tabacum and N. benthamiana miR390 complementary sites originate from primers used for the amplification of ta-siARF loci. GeneBank accession numbers of the sequences included in the alignment are as follows: Nicotiana tabacum, FJ804751; Nicotiana benthamiana, FJ804742; Arabidopsis thaliana, BX838290; Glycine max, BE330988; Manihot esculenta, CK652751; Malus domestica, CN490861; Vitis vinifera, DT025007; Populus trichocarpa, DT498974; Lycopersicon esculentum, DV105041.
