Novel miR390-Dependent Transacting siRNA Precursors in Plants Revealed by a PCR-Based Experimental Approach and Database Analysis
Figure 3
Nucleotide sequence alignment of “170-nt-long” ta-siARF loci of dicot plants. Sequences of Nicotiana tabacum, Datura stramonium, Solanum demissum, and Physalis longifolia (undelined) were determined in this study the others were from GeneBank. Boxes indicate miR390 complementary sites and ta-siARF-encoding sequence regions. The length of nonconserved sequence between the ta-siARF sequence and -miR390 complementary site not included in the alignment is indicated. Dashes in the sequences of Cichorium endivia, Citrus aurantium, Citrus sinensis, and Populus tremula indicate the sequence region lacking the -miR390 complementary site that could not be aligned with other sequences. Shading shows residues identical for the given position in most sequences. Sequences of N. tabacum, D. stramonium, S. demissum, and P. longifolia miR390 complementary sites originate from primers used for the amplification of ta-siARF loci. GeneBank accession numbers of the sequences included in the alignment are as follows: Nicotiana tabacum, FJ804743; Datura stramonium, FJ804744; Solanum demissum, FJ804745; Physalis longifolia, FJ804746; Nicotiana tabacum, AM791738; Solanum lycopersicum, BE459870; Vitis vinifera, CU775162; Beta vulgaris, CV301446; Manihot esculenta, DV447689; Gossypium hirsutum, DW502659; Helianthus exilis 1, EE630512; Helianthus exilis 2, EE657417; Cyamopsis tetragonoloba, EG977206; Vigna unguiculata, FG922881; Cynara scolymus, GE588140; Cichorium endivia, EL348656; Citrus aurantium, EY848024; Citrus sinensis, CK935773; Populus tremula, DN500355.
