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Journal of Biomedicine and Biotechnology
Volume 2009 (2009), Article ID 969480, 7 pages
http://dx.doi.org/10.1155/2009/969480
Research Article

Effect of Chlorotriphenyl Derivatives of Sn and Pb upon Biophysical Properties of Membranes

1Institute of Physics, Opole University, Oleska 48, 45-052 Opole, Poland
2Institute of Biotechnology and Molecular Biology, Opole University, ul. Kominka 4, 45-033 Opole, Poland

Received 11 February 2009; Revised 5 May 2009; Accepted 20 May 2009

Academic Editor: Rumiana Koynova

Copyright © 2009 Dariusz Man et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Biophysical activity of two twin organometallic compounds Triphenyltin chloride (TPhT) and Triphenyllead chloride (TPhL) in their interreaction with model membranes, as well as with yeast cells Saccharomyces cerevisiae, was investigated. Four measurement methods were used in the experiments: two physical methods (spin probes method and the electric method); two biological methods (minimal inhibitory concentration /MIC/ and yeast survival test). It has been found that the activity of TPhT in interaction with model membranes and yeast cells is distinctly greater than that of TPhL. The activity manifests itself by considerable increase in the fluidity of the middle part of liposome bilayer, change in the polarization direction of the transmembrane voltage of filters impregnated with lauric acid, and in complete inhibition of growth of yeast cells at the concentration of 5  𝜇 g / m L .