SAROTUP: Scanner and Reporter of Target-Unrelated Peptides

Table 1

Known patterns of target-unrelated peptides.

TUP Category	TUP Pattern	Mechanism in brief
Capturing agents	H-P-[QM], G-D-[WF]-x-F, W-x-W-L, E-P-	Binding to streptavidin
	D-W-[FY], D-V-E-x-W-[LIV]
	W-x-P-P-F-[RK]	Binding to biotin
	W-[TS]-[LI]-x(2)-H-[RK]	Binding to Protein A
	R-T-[LI]-[TS]-K-P, [LFW]-x-F-Q, W-I-S-	Binding to secondary antibody
	x(2)-D-W, Q-[LV]-[LV]-Q, RTYK
Constant region of antibody (the target)	S-S-[IL], GELVW, G-[LI]-T-D-[WY],	Binding to the Fc fragment
	[RHK]-P-S-P, P-S-P-[RK]
Screening solid phase	W-x(2)-W, WHWRLPS, F-H-x(2)-W	Binding to plastic
Contaminants in the target sample	F-H-E-x-W-P-[ST]	Binding to contaminant bovine serum albumin
	QSYP	Binding to contaminant bovine IgG
Phage mutation	HAIYPRH	Growing faster than other phages