Impact of Lentiviral Vector-Mediated Transduction on the Tightness of a Polarized Model of Airway Epithelium and Effect of Cationic Polymer Polyethylenimine

Figure 2

Effect of LV transduction on TER. Polarized 16HBE41o- cells were incubated with different MOIs and, 4 or 24 hours later, TER was measured. Mock is represented by cells incubated with medium only. As a positive control, cells were incubated with 12 mM EGTA for 30 minutes. TER values are shown as % of mock cells that were 523 ± 44 Ohm × cm². Data are expressed as means ± SD of three experiments. A: ^∗𝑃<.05 for EGTA versus mock. B: ^∗𝑃<.05 for EGTA, 100, 500, and 2000 MOIs versus mock.

(a)

(b)