Efficient Isolation of Cardiac Stem Cells from Brown Adipose
Figure 1
(a) Comparisons between different methods in nucleated cell yield within one hour’s digest ( for each group). $: no significant difference,: compared with all other groups in the same digest time. (b) Representative images of cell viability analysis. Newly isolated cells from rat brown adipose were stained with Propidium iodide and then analyzed using flow cytometry. A: Cell viability after 30 minutes digestion with collagenase IV, dispase II, and trypsin; B: cell viability after 45 minutes digestion with collagenase IV, dispase II, and trypsin; C: cell viability after 60 minutes digestion with collagenase IV, dispase II, and trypsin; D: Comparison of cell viabilities after digesting for different time with collagenase IV, dispase II, and trypsin, ( = 6 for each group).
