Efficient Isolation of Cardiac Stem Cells from Brown Adipose
Figure 4
(a) Immunostaining of differentiated cells for cardiac-specific antibodies. Differentiated cardiomyocytes-like cells were specifically stained with antiα-sarcomeric actinin A–D, and anticardiac troponin A–I antibodies; the transverse striation can be clearly observed under high magnification microcopy (D and I, 4 magnifications of red frame in C and G, resp.). Furthermore, no specific staining was obtained with the antiMyoD (data not shown) (Bar = 50 m). (b) Expression of cardiac-specific genes in the differented cells analyzed by RT-PCR.
