Efficient Isolation of Cardiac Stem Cells from Brown Adipose

Table 1

Correlation between digest time and cell yield when different enzymes were used for brown adipose digest.

Digest time—Cell yield
Time	Enzyme∗	Cell yield$	Cell viability	Viable cell yield$
30 minutes	D/IV/T	8.49  ±  0.57	93.47 ± 1.18%	7.94 ± 0.58
	IV/T	2.74 ± 0.39	93.79 ± 1.22%	2.57 ± 0.39
	IV	1.47 ± 0.19	94.66 ± 0.99%	1.39 ± 0.18
	I	0.18 ± 0.02	93.95 ± 1.05%	0.17 ± 0.02
	D	0.78 ± 0.07	94.96 ± 0.88%	0.74 ± 0.07
45 minutes	D/IV/T	11.65 ± 1.07	90.72 ± 1.38%	10.56 ± 0.98^#
	IV/T	4.26 ± 0.56	91.87 ± 1.34%	3.92 ± 0.54
	IV	1.82 ± 0.20	92.87 ± 1.69%	1.69 ± 0.20
	I	0.23 ± 0.04	91.96 ± 0.77%	0.22 ± 0.04
	D	0.87 ± 0.06	93.18 ± 1.36%	0.81 ± 0.07
60 minutes	D/IV/T	11.72 ± 0.86	86.88 ± 1.50%	10.19 ± 0.91
	IV/T	4.66 ± 0.33	90.86 ± 1.45%	4.23 ± 0.31#
	IV	1.96 ± 0.15	91.17 ± 1.55%	1.79 ± 0.14#
	I	0.28 ± 0.04	90.10 ± 1.14%	0.26 ± 0.04#
	D	1.02 ± 0.13	91.14 ± 1.57%	0.93 ± 0.12#

Nucleated cell yields of cell viability were compared among different enzymes and different digest time. Data are presented as mean ±  standard deviation of 𝑛 = 6 for each group.
∗D Dispase II; IV: collagenase IV; T: trypsin; I: collagenase I;
#Optimal cell yield for the enzyme.
$10⁶ cells/g tissue.