Efficient Isolation of Cardiac Stem Cells from Brown Adipose

Table 3

Comparison of differentiated cTnT+ cardiomyocytes (%) from brown adipose-derived cells at different seeding densities.

Cell density	5×10^4/cm^2	2.5×10^4/cm^2	1×10^4/cm^2	5×10^3/cm^2	2.5×10^3/cm^2	1×10^3/cm^2
Combination∗	24.78 ± 2.4	29.93 ± 3.2#	25.41 ± 2.23	21.43 ± 1.62	16.7 ± 1.41	5.3 ± 0.6
Dispase II∗	11.64 ± 1.98	15.43 ± 2.76	12.35 ± 2.21	9.64 ± 2.55	5.7 ± 1.24	2.41 ± 0.91

∗Combination: cells isolated by combination of collgenase IV, dispase II, and trypsin; Dispase II: cells isolated by dispase II alone.
#The optimal seeding density for brown adipose-derived cardiac stem cells differentiation into cardiomyocytes (𝑃<.01,𝑛 = 6).