Proteomics Strategy for Identifying Candidate Bioactive Proteins in Complex Mixtures: Application to the Platelet Releasate
Figure 5
Western blot validation of novel platelet proteins. 30 L of resting and TRAP-stimulated platelet releasate were separated on a 4%–20% gradient SDS-PAGE gel and probed for PEDF and involucrin. (a) PEDF was detected as a band at approximately 50 kDa, with additional heavier bands that may represent glycosylated forms of the protein. (b) Involucrin was detected at the expected molecular weight as a band resolving at approximately 120 kDa. The locations of molecular weight markers are also indicated. These proteins were identified in TRAP-stimulated platelet releasates from 4 individual donors. Images displayed are representative of the 4 individual donors. R = Resting/unstimulated platelet releasate T = TRAP-stimulated platelet releasate.
