Research Article

Proteomics Strategy for Identifying Candidate Bioactive Proteins in Complex Mixtures: Application to the Platelet Releasate

Table 2

Proteins identified in Fraction 27.

IPI no.Protein nameSpectral countOther fractions where protein was foundDescriptionPreviously found in platelets

IPI00745872.2Isoform 1 of serum albumin precursor27423–26, 28, 31, 42Serum albuminYes
IPI00022434.4Uncharacterized protein alb7822–26, 28–43,Albumin proteinYes
IPI00021439.1Actin, cytoplasmic 12022-23, 25-26, 28–29, 31–33Highly conserved cytoskeletal protein involved in cell motilityYes
IPI00298497.3Fibrinogen beta chain, precursor1823–26, 28, 44Yields monomers that polymerize fibrin and act as a cofactor in platelet aggregationYes
IPI00021891.5Isoform gamma-b of fibrinogen gamma chain precursor1423–26Yields monomers that polymerize fibrin and act as a cofactor in platelet aggregationYes
IPI00027547.2Dermcidin precursor158–26, 28–32, 34, 41, 45Antimicrobial activity and survival promoting peptide for neurons
IPI00884222.1Similar to keratin, type 1 cytoskeletal 106232Heterotetramer of two type I and two type II keratins
IPI00021841.1Apolipoprotein a-i precursor325, 31-32, 34, 44–45Participates in reverse transport of cholesterol from tissues to the liver
IPI00013933.2Isoform dpi of desmoplakin522, 24, 29–32High molecular weight protein of desmosomes
IPI00296099.6Thrombospondin-1 precursor3226, 28Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactionsYes
IPI00021885.1Isoform 1 of fibrinogen alpha chain precursor523–26, 34Cofactor in platelet aggregation and yield monomers that polymerize into fibrinYes
IPI00478003.1Alpha-2-macroglobulin precursor1323, 25, 44Protease inhibitor
IPI00022445.1Platelet basic protein precursor1223–26, 28–34Precursor for a number of peptides with antimicrobial (TC-1 and TC-2), chemoattractant, and stimulation of DNA synthesis activityYes
IPI00419424.3Igkv1-5 protein223–25, 31, 42, 44–45Immunoglobulin
IPI00453473.6Histone h4322-23, 25-26, 30–32, 34, 36Core component of nucleosomes playing a central role in DNA repair, replication, and transcription
IPI00783987.2Complement c3 precursor (fragment)1522, 25Involved in activation of the complement system and local inflammation
IPI00026314.1Isoform 1 of gelsolin precursor1426Calcium-regulated, actin-modulating proteinYes
IPI00022434.4Uncharacterized protein alb.7822–26, 28–43Uncharacterized albumin proteinYes
IPI00017601.1Ceruloplasmin precursor1522Glycoprotein involved in iron transport across cell membrane
IPI00382606.1Factor vii active site mutant immunoconjugate445Initiates extrinsic pathway of blood coagulation
IPI00154742.6Igl@ protein224-25, 31Immunoglobulin
IPI00550991.3Alpha-1-antichymotrypsin precursor644Inhibits neutrophil cathepsin G and mast cell chymase
IPI00025252.1Protein disulfide-isomerase a3 precursor426Catalyses the rearrangement of S-S bonds in proteinsYes
IPI00022431.1Alpha-2-hs-glycoprotein precursor344Secreted protein, part of the fetuin family, may be involved in differentiationYes
IPI00032292.1Metalloproteinase inhibitor 1 precursor3Complexes with metalloproteinases and irreversibly inactivates themYes
IPI00555812.4Vitamin d-binding protein precursor123, 44Multifunctional protein found in many body fluids and on the surface of various cell typesYes
IPI00179709.4Isoform 1 of tubulin alpha-3c/d chain2Major constituent of microtubules
IPI00006114.4Proliferation-inducing protein 35/PEDF2Induces neuronal differentiation and inhibits angiogenesisYes
IPI00739464.1Similar to cytoplasmic beta-actin131Highly conserved cytoskeletal protein involved in cell motilityYes
IPI00414860.660s ribosomal protein l37a1Ribosomal protein containing a zinc finger domain, expressed in many cell typesYes
IPI00020996.3Insulin-like growth factor-binding protein complex acid labile chain precursor1Secreted protein involved in protein complexes with insulin-like growth factor family
IPI00418471.6Vimentin1Class III intermediate filament protein found in many nonepithelial cellsYes