Isolation of Nebulin from Rabbit Skeletal Muscle and Its Interaction with Actin
Figure 5
Binding of purified nebulin and F-actin examined by cosedimentation. (a) nebulin only, (b) actin only, (c) nebulin + actin. (S) supernatant after centrifugation, (P) pellet after centrifugation. Nebulin and actin were mixed in 0.11 M KPO4, 0.31 M urea, 0.27 mM ATP, and 0.1 mM MgCl2, pH 7.0. Electrophoresis was performed on a 2–15% polyacrylamide gel.
