Isolation of Nebulin from Rabbit Skeletal Muscle and Its Interaction with Actin
Figure 7
Binding of thin filament proteins to purified nebulin by far western blot analysis. Proteins (0.2 g) adsorbed onto a nitrocellulose membrane were incubated in nebulin reaction solution (0.18 M KPO4, 0.1 mM MgCl2, 0.1 mM EGTA, and 0.5 M urea, pH 7.0) for 20 hours at 4°C. Amido black: amido black staining; +nebulin: incubation in nebulin (15 g/ml), −nebulin: incubation in reaction solution alone.
