ICAM-1 Clustering on Endothelial Cells Recruits VCAM-1
Figure 1
ICAM-1 and VCAM-1 localization in TNFα-stimulated endothelial cells. (a) TNFα-treated HUVECs were fixed and stained as indicated. Bar, 20 m. (b) Detailed analysis of ICAM-1 and VCAM-1 co-localization at the apical surface (arrowheads) in TNFα-treated endothelial cells that were fixed and stained as indicated. Bar, 10 m. (c) Differentiated HL60 cells were allowed to adhere to TNFα-treated HUVECs for 30 minutes, after which cells were fixed and stained as indicated. Apical focal plane shows that ICAM-1 and VCAM-1 (arrowheads) are recruited around adhered HL60 cells (asterisks). Bar, 10 m.