121947.fig.004a
(a)
121947.fig.004b
(b)
Figure 4: Standardization of ELISA for CRAMP. (a) The results show that the fresh plasma has the highest ELISA data, and the plasma kept in 4°C overnight or in the room temperature less than 3 hours, and the plasma thawed less than 3 times have little lower data, but the statistics does not show the significant difference. But the plasma kept in the room temperature more than 4 hours, thawed more than 4 times have the higher data, the statistics shows the significant difference. (b) The results show that the fresh plasma has the highest ELISA data, and the plasma kept in 4°C over 1 night, 2, 3, 4, 5 nights or in the room temperature for 1 day 2, 3, 4, 5 days have much lower data, and the statistics show the significant difference.