Research Article

Identification, Characterization, and Effects of Xenopus laevis PNAS-4 Gene on Embryonic Development

Figure 2

Cellular localization, and temporal and spatial expression patterns of xPNAS-4. (a) HEK293 cells were transfected with pEGFP-N1 or pEGFP-N1-xPNAS-4 plasmids as explained in materials and methods. Left: pEGFP-N1 transfected cells ( 2 0 0 × ) ; Right: pEGFP-N1-xPNAS-4 transfected cells ( 2 0 0 × ) . (b) Analysis of the temporal expression pattern of xPNAS-4 gene by semiquantitative RT-PCR. ODC: X. laevis ornithine decarboxylase as internal control gene. RT (–): control reverse transcription without template. (c) Whole mount in situ hybridizations showing the spatial expression pattern of xPNAS-4 gene. The upper-left image shows the expression pattern of the xPNAS-4 gene in a stage-2 embryo. The upper-right image shows the expression pattern of the xPNAS-4 gene in a stage-10 embryo (beginning of gastrulation). An ubiquitous expression pattern (purple staining) can be seen. The lower image shows the expression pattern of the xPNAS-4 gene at stage 35. xPNAS-4 transcripts were present in the head, dorsal neural tissue, and prospective heart.
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