Identification, Characterization, and Effects of Xenopus laevis PNAS-4 Gene on Embryonic Development
Figure 5
Effect of xPNAS-4 MOs microinjection. (a) Embryos microinjected with 20 ng of xPNAS-4 MO exhibited failure of head development and shortened axis defects (left), but embryos microinjected with 20 ng of control MO embryos were normal (right). (b) The embryos were partially rescued by co-injected with 450 pg of xPNAS-4 mRNA and 20 ng of xPNAS-4 MO (the second row) in comparison with embryos microinjected with 20 ng of xPNAS-4 MO alone (the first row). Better rescue was observed after co-injection of 20 ng of xPNAS-4 MO plus 900 pg of xPNAS-4 mRNA (the third row). The fourth row shows a normal embryo microinjected with 20 ng of control MO. (c) The rate of defective embryos seen in the experiment.
