Insulin Promotes Survival of Amyloid-Beta Oligomers Neuroblastoma Damaged Cells via Caspase 9 Inhibition and Hsp70 Upregulation
Figure 3
Effects of insulin against rA42 oligomers on caspase 9 and 3 activation. LAN5 were untreated (control), treated with rA42 oligomers (40 M) for 1 h (rA42), rA42 oligomers (40 M) for 1 h and insulin (100 M) for 4 h (rA42 + insulin), insulin alone (100 M) for 4 h (insulin), 50 M Vinblastine for 4 h (activator) and submitted to caspase 8 (a), caspase 9 (b), and caspase 3 (c) luminescent assays. Data expressed as percentage of the corresponding control. The dashed line shown in the histogram corresponds to a control culture. The activation of caspase 8 (a) is significantly high with activator , whereas it is not significantly high in rA42 versus control. The activation of caspase 9 (b) is significantly high in rA42 and in the activator , whereas it is not significantly high in rA42 + insulin versus control. The activation of caspase 3 (c) is significantly high in rA42 and in the activator , whereas it is not significantly high in rA42 + insulin versus control. Values are referred to control, the data are the mean SD of three separate experiments.