Upregulation of Endogenous HMOX1 Expression by a Computer-Designed Artificial Transcription Factor

<table>Effect of the ATF on endogenous HMOX1 expression. Quantitative real-time RT-PCR was performed; the candidate vectors were pcDNA3.1-AZP-p65-flag (AZP-p65), pcDNA3.1-NLS- AZP-p65-flag (NLS-AZP-p65), PUC57-AZP (AZP), pcDNA3.1-NLS-eGFP-p65-flag (p65), and pcDNA3.1-NLS-<svg height="14.925" id="M62" style="vertical-align:-2.72116pt" version="1.1" viewbox="0 0 28.1625 14.925" width="28.1625" xmlns="http://www.w3.org/2000/svg">
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</svg>-p65). For each of them, four dose gradients (0.5 <svg height="9.6750002" id="M64" style="vertical-align:-2.29482pt" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg">
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</svg>g, 1.5 <svg height="9.6750002" id="M66" style="vertical-align:-2.29482pt" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg">
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</svg>g, and 2.0 <svg height="9.6750002" id="M67" style="vertical-align:-2.29482pt" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg">
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</svg>g) were used. The data represent the means ± S.D.</table>

BioMed Research International

fig6

Figure 6

Figure 6: Upregulation of Endogenous HMOX1 Expression by a Computer-Designed Artificial Transcription Factor 