Upregulation of Endogenous HMOX1 Expression by a Computer-Designed Artificial Transcription Factor

Table 1

Details of the 12 possible target sites for the AZP.

Target	Score (Base function)	TSO Issues
(1) 5^-GGA GGG GGG ACT CGC GGA-3^	52.95	Yes
(2) 5^-GGG GGG ACT CGC GGA AAC-3^	56.51	Yes
(3) 5^-GGG ACT CGC GGA AAC AAA-3^	56.18	Yes
(4) 𝟓^′ -ACT CGC GGA AAC AAA GGG- 𝟑^′	56.18	No
(5) 5^-CGC GGA AAC AAA GGG AAG-3^	54.35	Yes
(6) 5^-GGA AAC AAA GGG AAG GCG-3^	52.08	Yes
(7) 5^-AAC AAA GGG AAG GCG GAT-3^	55.03	Yes
(8) 5^-GCG GAA ACA AAG GGA AGG-3^	52.26	No
(9) 5^-GAA ACA AAG GGA AGG CGG-3^	54.03	No
(10) 5^-ACA AAG GGA AGG CGG ATT-3^	54.43	No
(11) 5^-CGG AAA CAA AGG GAA GGC-3^	54.19	No
(12) 5^-AAA CAA AGG GAA GGC GGA-3^	51.03	No