DNA Vaccines: Developing New Strategies against Cancer
Table 1
DNA vaccines main features.
Advantage
Drawback
Design
Allowing the introduction of several immunological components; synthetic and PCR methods for simple modifications
Low transfection efficiency
Manufacture
Rapid production and formulation; easily engineered, reproducible, large-scale production and isolation
Safety
No pathogenic infection in vivo; no significant adverse events in any clinical trial; neutralizing immune responses rarely observed; boost strategy is possible
Stability
Long shelf life; relative temperature insensitivity; lack necessity of a cold chain stored on a large scale
Immunogenicity
Lower immunogeni-city in larger animals and human compared to mice