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Journal of Biomedicine and Biotechnology
Volume 2010 (2010), Article ID 179817, 6 pages
http://dx.doi.org/10.1155/2010/179817
Research Article

Histochemical Evaluation of Human Prostatic Tissues with Cratylia mollis Seed Lectin

1Laboratório de Glicoproteínas, Departamento de Bioquímica, Universidade Federal de Pernambuco (UFPE), 50670-901 Recife, PE, Brazil
2Laboratório de Imunopatologia Keizo Asami (LIKA), UFPE, 50670-901 Recife, PE, Brazil

Received 26 August 2009; Revised 16 March 2010; Accepted 13 April 2010

Academic Editor: Richard D. Ludescher

Copyright © 2010 Amanda L. R. de Lima et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Lectins, proteins which selectively recognize carbohydrates, have been used in histochemistry for the evaluation of changes in glycosylation in processes of cellular differentiation and/or dedifferentiation. Cratylia mollis seed lectins (Cramoll 1,4 and Cramoll 3), conjugated to horseradish peroxidase, were used as histochemical probes in human prostate tissues: normal (NP), hyperplasia (BPH), and prostate carcinoma (PCa). The staining pattern of Con-A and Cramoll 1,4 in BPH was more intense than in NP. These lectins also showed staining differences between BPH and PCa; the latter showing decreased staining intensity with an increased degree of malignancy. PNA and Cramoll 3 stained epithelial cells similarly in all diagnoses although they did present intense staining of PCa glands lumen. Corpora amylacea were not differentially recognized by any of the lectins. Cramoll 1,4 and Cramoll 3 seed lectins present themselves as candidates for histochemical probes for prostate pathologies when compared to commercial lectins such as Con-A and PNA.