Histochemical Evaluation of Human Prostatic Tissues with Cratylia mollis Seed Lectin
Figure 2
Histochemistry of prostatic tissues using Con A, Cramoll 1,4, PNA, and Cramoll 3. (a) Normal prostate stained with Con-A. Epithelial cells (arrow) present a weak staining while stroma (asterisk) is intensely stained. (b) Moderate staining by Cramoll 1,4 in epithelium of normal glands. (c and d) Normal prostate epithelium and stroma (asterisks) presenting weak staining by PNA and Cramoll 3, respectively. (e) Con A moderate staining of BPH glands (arrow). (f) BPH epithelial cells showing intense staining by Cramoll 1,4 in apical cytoplasm (arrowheads) and membrane (arrow). (g and h) BPH glands (arrows) were not stained or only weakly stained by PNA and Cramoll 3, respectively. (i and j) Gleason score 2 and 3 PCa cells stained with a moderate pattern, respectively. (k) Weak staining in Gleason score 4 PCa cells. (l) Nonstaining cells in Gleason score 5 PCa. (m and n) PCa gland lumens (arrows) presented an intense staining pattern for PNA and Cramoll 3, respectively. Magnification (a, b, c, d, k, m, and n) x100 and (e, f, g, h, i, j, and l) x400.
