Research Article

BALB/c Mice Vaccinated with Leishmania major Ribosomal Proteins Extracts Combined with CpG Oligodeoxynucleotides Become Resistant to Disease Caused by a Secondary Parasite Challenge

Table 1

Cytokine production by popliteal DLN cells from vaccinated reinfected mice at week seven after secondary challenge.

SLALRPMedium

IFN- š›¾ 5 8 3 7 . 1 6 Ā± 8 3 4 . 8 2 5 7 7 3 . 5 2 Ā± 1 4 1 1 , 1 4 1 6 3 5 , 1 1 Ā± 6 0 7 , 8 9
IL-10 5 2 6 . 3 1 Ā± 2 1 4 . 9 8 4 0 8 , 9 2 Ā± 2 3 3 . 3 6 1 0 4 , 3 9 Ā± 5 7 , 1 3
IL-4 2 2 9 . 5 4 Ā± 5 8 . 7 8 4 4 . 3 6 Ā± 3 1 . 2 2 5 4 . 3 8 Ā± 3 7 . 8 9

The level of cytokines was determined by ELISA in the supernatant of popliteal DLN cells obtained from reinfected mice at week seven post rechallenge, after in vitro stimulation with 12ā€‰ šœ‡ g/ml of SLA and LRP. MeanĀ±SD of samples from six mice is shown (pg/ml).