Research Article
Genotoxic and Cytotoxic Safety Evaluation of Papain (Carica papaya L.) Using In Vitro Assays
Table 1
(a) Effect of different papain concentrations on the survival of E. coli strains. Exponentially growing cultures were centrifuged, washed with 0.9% NaCl sterile solution, and suspended in the same solution. Aliquots (1 mL) of these suspensions were incubated with different papain concentrations or 0.9% NaCl for 60 minutes, at 37C, with shaking. Afterwards, aliquots (100 L) were taken, diluted and plated onto LB medium for determining surviving fractions () for each strain at different papain concentrations. Values are the mean of 3 independent experiments (6 determinations) with standard deviations not exceeding 15% (mean ± SD). 5% Significance level was adopted to compare data. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The results are not significantly different when compared to negative control. * The results are significantly different when compared to negative control. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
(b) Effect of papain associated with talc on the survival of E. coli strains. Exponentially growing cultures were centrifuged, washed with 0.9% NaCl, and suspended in the same solution. Aliquots (1 mL) of these suspensions were incubated with different papain concentrations (associated or not with talc) or 0.9% NaCl for 60 minutes, at 37C, with shaking. Afterwards, aliquots (100 L) were taken, diluted and plated onto LB medium for determining surviving fractions () for each strain. Values are the mean of 3 independent experiments (6 determinations) with standard deviations not exceeding 15% (mean ± SD). 5% significance level was adopted to compare data. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The results are not significantly different when compared to negative control. * The results are significantly different when compared to negative control. |