Figure 4: Effect of Cry1Ac on serum cytokines in Plasmodium-infected mice.Mice were treated with Cry1Ac protoxin or PBS once weekly for four weeks. One day after the last injection, mice were infected with P. berghei ANKA or P. chabaudi AS. On days 4 and 8 PI (a and c) or days 8 and 18 PI (b and d), three mice from each group were killed, their sera was separated from peripheral blood and the levels of the cytokines IFN- (a and b) and IL-4 (c and d) were measured by cytometric bead array. Data are representative of two separate experiments. Asterisks indicate statistically significant differences between the indicated groups.