Figure 4: In vitro induction of polarization and apoptosis in T cells derived from normal mice with SWA and SEA. T cells were purified from normal mice by MACS and preactivated overnight with anti-CD3 (2 g/mL) and anti-CD28 (1 g/mL) antibodies. Then the cells were stimulated with specific antigens of SEA, SWA or PBS alone for 36 hours at C in 5% , followed by staining with rabbit antimouse caspase-3 antibody or rabbit IgG isotype control antibody plus anti-CD4-FITC, anti-IFN--PE anti-IL-4-PE mAbs, or isotype control antibodies prior to FACS analysis. The percentage of apoptotic cells in the FACS data was derived from the number of cells that were and IFN- or and IL- and gated on the caspase- population. Data are expressed as the meanSD of 18 mice from three independent experiments. ; . Upper panels: One representative experiment of flow cytometric analysis with the average percentage of Th or apoptotic cells shown in the FACS data. Lower panels: The statistical analysis of 18 mice from three independent experiments.