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Journal of Biomedicine and Biotechnology
Volume 2010 (2010), Article ID 212817, 8 pages
http://dx.doi.org/10.1155/2010/212817
Research Article

Excreted/Secreted Proteins from Trypanosome Procyclic Strains

1UMR 177, IRD-CIRAD, CIRAD TA A-17 / G, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France
2UMR, CNRS 5554, Institut des Sciences de l'Evolution, Université Montpellier II, 34095 Montpellier Cedex 5, France
3Organisation des Nations Unies, INRA, UR1199, LPF; 2 place Pierre Viala Bât., 13 34060 Montpellier Cedex 01, France
4UFR Odontologie, EA 4203, 545 Avenue du Pr Viala, Université Montpellier I, 34193 Montpellier Cedex 5, France

Received 22 July 2009; Accepted 19 September 2009

Academic Editor: Luis I. Terrazas

Copyright © 2010 Celestine Michelle Atyame Nten et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Trypanosoma secretome was shown to be involved in parasite virulence and is suspected of interfering in parasite life-cycle steps such as establishment in the Glossina midgut, metacyclogenesis. Therefore, we attempted to identify the proteins secreted by procyclic strains of T. brucei gambiense and T. brucei brucei, responsible for human and animal trypanosomiasis, respectively. Using mass spectrometry, 427 and 483 nonredundant proteins were characterized in T. brucei brucei and T. brucei gambiense secretomes, respectively; 35% and 42% of the corresponding secretome proteins were specifically secreted by T. brucei brucei and T. brucei gambiense, respectively, while 279 proteins were common to both subspecies. The proteins were assigned to 12 functional classes. Special attention was paid to the most abundant proteases (14 families) because of their potential implication in the infection process and nutrient supply. The presence of proteins usually secreted via an exosome pathway suggests that this type of process is involved in trypanosome ESP secretion. The overall results provide leads for further research to develop novel tools for blocking trypanosome transmission.